Plants are a important source of food, but also produce metabolites that are used to treat diseases. One class of those metabolites are monoterpene indole alkaloids (MIAs). We participated in MIAMi a Horizon 2020 project to identify and transfer the metabolic pathways from plants to yeast. We sequenced, assembled, and annotated 14 plant genomes using the nanopore sequencing platform. Several papers were published as a results of this project.
- Lezin E, Durand M, Birer Williams C, Lopez Vazquez AL, Perrot T, Gautron N, Pétrignet J, Cuello C, Jansen HJ, Magot F, Szwarc S, Le Pogam P, Beniddir MA, Koudounas K, Oudin A, St-Pierre B, Giglioli-Guivarc’h N, Sun C, Papon N, Jensen MK, Dirks RP, O’Connor SE, Besseau S, Courdavault V. Genome-based discovery of pachysiphine synthases in Tabernaemontana elegans. Plant J. doi: 10.1111/tpj.17085. Free pdf
- Holtz M, Rago D, Nedermark I, Hansson FG, Lehka BJ, Hansen LG, Marcussen NEJ, Veneman WJ, Ahonen L, Wungsintaweekul J, Acevedo-Rocha CG, Dirks RP, Zhang J, Keasling JD, Jensen MK. Metabolic engineering of yeast for de novo production of kratom monoterpene indole alkaloids. Metab Eng. S1096-7176(24)00128-9. Free abstract
- Lezin E, Carqueijeiro I, Cuello C, Durand M, Jansen HJ, Vergès V, Williams CB, Oudin A, De Bernonville TD, Petrignet J, Celton N, St-Pierre B, Papon N, Sun C, Dirks RP, O’Connor SE, Jensen MK, Besseau S, Courdavault V. A chromosome-scale genome assembly of Rauvolfia tetraphylla facilitates the identification of the complete ajmaline biosynthetic pathway. Plant Commun. 5(4):100784. Free pdf
- Cuello C, Jansen HJ, Abdallah C, Zamar Mbadinga DL, Birer Williams C, Durand M, Oudin A, Papon N, Giglioli-Guivarc’h N, Dirks RP, Jensen MK, O’Connor SE, Besseau S, Courdavault V. The Madagascar palm genome provides new insights on the evolution of Apocynaceae specialized metabolism. Heliyon 10(6):e28078. Free pdf
- Stander EA, Lehka B, Carqueijeiro I, Cuello C, Hansson FG, Jansen HJ, Dugé De Bernonville T, Birer Williams C, Vergès V, Lezin E, Lorensen MDBB, Dang TT, Oudin A, Lanoue A, Durand M, Giglioli-Guivarc’h N, Janfelt C, Papon N, Dirks RP, O’connor SE, Jensen MK, Besseau S, Courdavault V. The Rauvolfia tetraphylla genome suggests multiple distinct biosynthetic routes for yohimbane monoterpene indole alkaloids. Commun Biol. 6(1):1197. Free pdf
- Cuello C, Stander EA, Jansen HJ, Dugé de Bernonville T, Oudin A, Birer-Williams C, Lanoue A, Giglioli-Guivarc’h N, Papon N, Dirks RP, Jensen MK, O’Connor SE, Besseau S, Courdavault V. An updated version of the Madagascar periwinkle genome. F1000Research 11-1541/v1. Free pdf
- Cuello C, Stander EA, Jansen HJ, Dugé de Bernonville T, Lanoue A, Giglioli-Guivarc’h N, Papon N, Dirks RP, Jensen MK, O’Connor SE, Besseau S, Courdavault V. Genome assembly of the medicinal plant Voacanga thouarsii. Genome Biol Evol: evac158. Free pdf
- Stander EA, Cuello C, Birer-Williams C, Kulagina N, Jansen H, Carqueijeiro I, Méteignier LV, Vergès V, Oudin A, Papon N, Dirks R, Jensen MK, O’Connor SE, Dugé de Bernonville T, Besseau S, Courdavault V. The Vinca minor genome highlights conserved evolutionary traits in monoterpene indole alkaloid synthesis. G3 (Bethesda) 12(12): jkac268. Free pdf
Image of Rauvolfia tetraphylla by Vengolis
